Benkovskaya G.V.1, Savchenko R.G.2 2019. [In vitro test with house fly (Musca domestica) larvae for evaluation of 20-hydroxyecdysone activity] // Euroasian Entomological Journal. Vol.18. No.4: 274–276 [in Russian, with English summary].
1 Institute of Biochemistry and Genetics, Ural Scientific Center, Russian Academy of Sciences, Oktyabrya Ave. 71, Ufa 450054 Russia. E-mail: bengal2@yandex.ru
2 Institute of Petrochemistry and Catalysis, Russian Academy of Sciences, Oktyabrya Ave. 141, Ufa 450075 Russia. E-mail: rimasavchenko@mail.ru
doi: 10.15298/euroasentj.18.4.05
ABSTRACT. The procedure of modified test for evaluation of ecdyson-like activity of 20-hydroxyecdysone is presented. The modification includes exposure of cuticle-muscle tissues of house fly larvae in the medium with added compounds under test instead of ligated larva. Procedure of sample preparation and registration of test results allows reducing the screening time and standardizing the quantitative assessment of activity of compounds.
KEY WORDS: 20-hydroxyecdysone, house fly, test in vitro.