Burdina E.V.1, Kerchev I.A.2,3, Smirnov N.А.2, Gruntenko N.E.1 2022. [Effect of heat stress on alkaline phosphatase activity in bark beetles Ips amitinus (Eichhoff, 1872) and Polygraphus proximus Blandford, 1894 (Coleoptera, Curculionidae: Scolytinae)] // Euroasian Entomological Journal. Vol.21. No.5: 258–264 [in Russian, with English summary].
1 Institute of Cytology and Genetics SB RAS, Рrosp. Akademika Koptuga 2, Novosibirsk 630090 Russia. E-mail: bella79@list.ru
2 Institute of Monitoring of Climatic and Ecological Systems SB RAS, Akademicheskii Prosp. 10/3, Tomsk 634055 Russia.
3 Institute of Systematic and Ecology of Animals, Russian Academy of Sciences, Siberian Branch, Frunze Str. 11, Novosibirsk 630091 Russia.
doi 10.15298/euroasentj.21.5.02
ABSTRACT. Alkaline phosphatases are widespread in nature and can be found in various groups of organisms. In insects, this enzyme is involved in synthesis regulation of dopamine, an element of the neuroendocrine system, which controls behaviour, reproduction and adaptation. This research is devoted to the study of the alkaline phosphatase activity in imagoes of two economically important bark beetle species alien to Western Siberia, Ips amitinus (Eichh.) and Polygraphus proximus Blandf. Enzyme activity was measured under normal conditions and heat stress (+38°C) of various durations. It was found that an hour into the heat exposure, the activity of alkaline phosphatase increased in individuals of both species. However, the amplitude of the heat stress response in P. proximus was significantly higher than in I. amitinus, which could probably be explained by differences in the choice of microhabitat.
KEY WORDS: Alkaline phosphatase, heat stress, Ips amitinus, Polygraphus proximus.