Obukhova A.L., Khabarova M.Yu., Voronezhskaya E.E. 2017. Selective visualization of monoamine uptake and synthesis system in sea urchin larvae Paracentrotus lividus (Lamarck, 1816) // Invertebrate Zoology. Vol.14. No.2: 162–166 [in English].
Institute of Developmental Biology, RAS, Vavilov st. 26, 119991, Moscow, Russia. E-mails: allobukhova@gmail.com , m.khabarova@yandex.ru , lenavor@gmail.com
ABSTRACT: We tested a method for the early visualization of neuronal elements in the larvae of sea urchin Paracentrotus lividus. Application of external serotonin (5-HT) and dopamine (DA) led to an increase in the mediator content within a specific set of neurons capable to 5-HT and DA uptake. Application of biochemical precursors 5-HTP and L-DOPA resulted in non-selective mediator increase within the most neurons of the larval body. In addition, primary mesenchyme cells which participate in the formation of spicules appeared to synthesize 5-HT from the precursor. Applied method of 5-HT and DA enhancement allows to visualize larval neurons long before they become labeled by routine immunochemical and hystochemical methods.
doi: 10.15298/invertzool.14.2.10
KEY WORDS: serotonin, dopamine, sea urchin larvae, neuronal elements.