Smirnov D.G., Titov S.V., Baishev F.Z. 2016. New microsatellite markers for Myotis daubentonii and Eptesicus nilssonii (Vespertilionidae, Chiroptera) // Russian J. Theriol. Vol.15. No.2: 81–83 [in English].
Dmitriy G. Smirnov [email@example.com], Sergey V. Titov, Farid Z. Baishev, Department of Zoology and Ecology, Penza State University, Krasnaja str. 40, Penza 440026, Russia.
ABSTRACT. New microsatellite markers were developed and characterized for two vespertilionid species: three loci for Myotis daubentonii (MmCCA, MdTTA, MdTTTA) and two loci for Eptesicus nilssonii (EnTCA, EnTCTA). The usefulness of these markers were assessed by screening a sample of wing membrane biopsy of 136 specimens of M. daubentonii and 206 specimens of E. nilssonii, collected in Samara Province, Penza Province and Khanty-Mansi Autonomous Area (Russia). All the loci were highly polymorphic. Both moderately and highly polymorphic loci were identified with 3-15 alleles segregating per locus (mean in M. daubentonii 3.45±0.29, in E. nilssonii 6.07±0.38). The minimal heterozygosity was found in locus MmCCA (HO=0.284±0.034, HE=0.305±0.035), while all the rest of loci had moderate and high heterozygosity. These polymorphic markers will provide a valuable tool for assessment of population genetics of these two species.
KEY WORDS: bats, Chiroptera, microsatellite, Myotis daubentonii, Eptesicus nilssonii.